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IRCT20230612058457N9 IRCT 2026-05-23 Vasile Alecsandri University Comparison of the sequencing of plyometric exercises and dynamic stretching on athletic and neuromuscular performance in soccer players Acute effects of plyometric-dynamic stretching sequencing on athletic and neuromuscular performance in soccer players: A randomized crossover trial 2026-03-16 anticipated 20 Complete https://irct.ir/trial/90268 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Not used, Assignment: Crossover, Purpose: Supportive, Randomization description: In this randomized crossover trial with three experimental conditions (DS+DJ, DJ+DS, and CC), the randomization sequence was generated by an independent researcher using randomizer.org software employing a balanced Latin square design to ensure that each condition appeared equally often in the first, second, and third positions across participants. The unit of randomization was individual, and no stratification was used. For allocation concealment, the generated sequences were placed in sequentially numbered, opaque, sealed envelopes, which were opened by the intervention deliverer only after completion of the standard warm-up and immediately before delivery of the experimental intervention. It should be noted that no quasi-randomization methods (such as allocation based on birth date or odd/even numbers) were used in this study, Blinding description: Due to the nature of the exercise interventions, participants and the intervention deliverer could not be blinded to condition allocation. However, outcome assessors responsible for administering performance tests and recording measurements were blinded to condition allocation, and data analysts were also blinded until the completion of the primary statistical analysis. Therefore, this study is considered double-blind. N/A Athletic Performance. Intervention 1: Intervention group: After 5 minutes of standard warm-up, the participant first performed dynamic stretching exercises consisting of two leg swing movements (anterior-posterior and medial-lateral), each with 15 repetitions per leg at a controlled rhythmic pace. Immediately after that, they performed three vertical drop jumps from a plyometric box at an individualized height between 30 and 50 cm. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Intervention 2: Intervention group: After 5 minutes of standard warm-up, the participant first performed three vertical drop jumps from a plyometric box at an individualized height between 30 and 50 cm. Immediately after that, they performed dynamic stretching exercises consisting of two leg swing movements (anterior-posterior and medial-lateral), each with 15 repetitions per leg at a controlled rhythmic pace. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Intervention 3: Control group: After 5 minutes of standard warm-up, the participant rested passively in a seated position for 5 minutes with no additional dynamic stretching or plyometric exercises. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Yes - There is a plan to make this available What will be shared: All data will be shared after anonymizing individuals, ensuring their non-identifiability. When: The data access period will start immeduately after the results are printed. To whom: The data will be made available to researchers, organizations, and institutions related to the field of football and also for publication in accessible journals. Conditions: In order to assist scientific research and promote the implementation of executive goals with a focus on reducing musculoskeletal injuries and enhancing the performance of football players in the field. Where to obtain: Mohammad Alimoradi/malimoradisport@gmail.com How to obtain: Requests for access to data will be answered within a week Comments:
public Mohammad Alimoradi
Unit 6, Yekta residential complex, Fathalishahi 12 St, Kerman, Iran
Kerman Iran (Islamic Republic of) 7614816961 +98 34 2250 1685 malimoradisport@gmail.com Shahid Bahonar University scientific Mohammad Alimoradi
Unit 6, Yekta residential complex, Fathalishahi 12 St, Kerman, Iran
Kerman Iran (Islamic Republic of) 7614816961 +98 34 2250 1685 malimoradisport@gmail.com Shahid Bahonar University Iran (Islamic Republic of) Male soccer players aged 18 to 25 years Competed at the university or competitive amateur club level Completed a minimum of three training sessions and one match per week Played in one of the following field positions: forward, midfielder, or full-back Had at least six months of structured soccer training Reported no lower limb injuries in the preceding three months 18 years 25 years Male Being a goalkeeper Being a central defender (due to distinct physical demands) Having current musculoskeletal injuries Using performance-enhancing supplements (e.g., creatine, beta-alanine, anabolic agents) Being unable to complete all testing sessions Having any medical condition that contraindicated maximal exercise N/A N/A N/A Intervention group: After 5 minutes of standard warm-up, the participant first performed dynamic stretching exercises consisting of two leg swing movements (anterior-posterior and medial-lateral), each with 15 repetitions per leg at a controlled rhythmic pace. Immediately after that, they performed three vertical drop jumps from a plyometric box at an individualized height between 30 and 50 cm. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Intervention group: After 5 minutes of standard warm-up, the participant first performed three vertical drop jumps from a plyometric box at an individualized height between 30 and 50 cm. Immediately after that, they performed dynamic stretching exercises consisting of two leg swing movements (anterior-posterior and medial-lateral), each with 15 repetitions per leg at a controlled rhythmic pace. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Control group: After 5 minutes of standard warm-up, the participant rested passively in a seated position for 5 minutes with no additional dynamic stretching or plyometric exercises. Then, 2 minutes of passive rest was given, followed by the performance testing battery. Agility. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: Timing gates (Speedlight, Swift Performance, Australia) placed at hip height (0.7 m) to align with the center of mass at the start/finish line. The participant began from a stationary two-point staggered stance with the front foot placed 0.5 m behind the start gate to prevent premature triggering. Time was recorded in seconds. Participants completed two maximal efforts with 2 minutes of passive recovery between attempts. The best (fastest) completion time was recorded. Repetetive speed ability total time. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: Six bouts of 10-meter sprints (including 5 meters straight and 5 meters with a 45° cut) with 25 seconds of passive recovery between each bout. Standing start. Timing gates (Speedlight, Swift Performance, Australia) were positioned at the start line (0 m) and the finish line (10 m total distance). Total time was calculated as the sum of all six sprint times and recorded in seconds. Repetetive speed ability fatigue index. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: Calculated using the formula: [(Total time / Ideal time) - 1] × 100. Ideal time was defined as the fastest sprint time multiplied by six. The result was reported as a percentage (%). 10m linear sprint. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: Timing gates were positioned at the start line and the 10-meter mark. The participant began from a stationary upright two-point staggered start with the front foot placed 0.5 m behind the start gate to prevent premature triggering. Time was recorded in seconds. Participants completed two maximal attempts with 2 minutes of passive recovery between trials. The best (fastest) time was recorded. Countermovement jump height. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: An iPhone 15 running the My Jump 3 application (version 3.0.10, Carlos Balsalobre, Madrid, Spain) was used. Videos were recorded at 240 frames per second using the iPhone 15's slow-motion capture mode from the sagittal plane. The participant stood upright with hands placed on the hips to eliminate arm swing assistance. The movement consisted of a rapid, maximal vertical jump preceded by a quick knee bend to approximately 90° of flexion, performed in a single seamless motion. Jump height was calculated using the formula h = t² × 1.22625, where h is jump height in meters and t is flight time in seconds. Three maximal attempts were performed with 30 seconds of passive recovery between each attempt. The best (highest) jump height was recorded in centimeters. Standing broad jump distance. Timepoint: Pre-test (before intervention) and post-test (immediately after 2 minutes of rest following the intervention). Method of measurement: The participant performed a horizontal jump from a standing start with a bilateral takeoff and landing. Arm swing was permitted. Landing distance was measured from the takeoff line to the nearest point of contact (heel) using a standardized measuring tape in centimeters. Three maximal attempts were performed, and the best distance was recorded. Vasile Alecsandri University Approved 2026-02-09 Ethics Committee of Vasile Alecsandri University of Bacău Calea Mărășești 157, 600115 Bacău, Romania Bacău Bacău Romania