Comparison of the effect of metanolic green tea، green coffee extract and chlorhexidine and on the salivary Streptococcus mutans ُin preschool children with early childhood caries by the PCR

Determination the effect of metanolic green tea، green coffee extract and chlorhexidine on Streptococcus mutans in preschool children with early childhood caries by the PCR

In this clinical trial study, there will be 90 children aged 4-6 who will be matched for demographic data. Children are randomly divided into 3 groups of 30 people and assigned a code to each participants. --------------------------------------------------------------------------------

ّFirst explain aims to parents, thenwritten informed consent is taken. A questionnaire including the demographic data of the child (age, sex, history of drug use, history of systemic disease, etc.) is also completed.Then, how to observe oral hygiene and brushing explain to all parents and wanted brush without toothpaste to their children for 2 days.During study, dont use gums containing Xylitol and any type of mouthwashes containing chlorhexidine and flouride. Upon completion of the two-day period, Which is considered as a washout period, children are randomly divided into 3 groups of 30 people. After taking saliva sample,too determining Streptococcus mutans, transfer samples to labrator

The entering criteria including children without any sysytemic disease and during the past month dont take antibiotic and topical flouride and dont have any orthodontic appliance. dont take gums containing Xylitol and any mouthmoush or gel containing flouride at least 3 weeks before study, dont have any soft tissue lesion in their mouth and do not have active and severe periodontal disease.

Chlorhexidine, Green Tea and Green Coffee

Streptococcus mutans

Simple randomization or unrestricted method will be used and randomization unit is Individual .we will use from Roll of a die from the methods of simple randomization because there are 3 groups in this study that actually the tool of randomization is dice. So the numbers 1 and 2 are for the first group (chlorhexidine) 3 and 4 for the second group (green coffee) and 5 and 6 for the third (green tea). The next step Allocation concealment will be done, In such a way that the allocated group is not known before the assignment of the individual. To achieve this goal, a non-transparent, sealed, random sequence envelope is used . In this way, the statistical specialist will provide 90 envelopes and each of the randomly generated links will be recorded on a card and the cards are arranged in the envelopes . In order to maintain a random sequence, the numbering of envelopes on the outer surface is done in the same order. Finally, the envelops are enclosed and placed in the box, respectively. At the time of the implementation of the study, according to the order of entry of eligible participants, one of the envelopes is opened respectively and will determine the group assigned to that participant. In this study, the assistant of Pediatric Dentistry examines the participants in terms of inclusion and exclusion criteria. Randomization methods are done by an statistical specialist and performance of the study is done by a trained dental student.

Because all the materials are in the same consistency gel, in a uniform shape and color, considering the informed consent, participants are unaware of material. Outcome evaluator, who performs PCR, is also unaware of the groups.

After this study, data and statistical analysis data are available to all.

After completing the research plan for 5 years of information dissemination

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