Study aim: The aim of this study is to determine the effect of digital storytelling-based education on the knowledge and attitudes of non-medical students toward human papillomavirus and its vaccination. Design: This is a quasi-experimental pretest-posttest study with a control group and two parallel intervention groups. Participants are allocated by block randomization with variable block sizes and equal allocation ratio, stratified by gender and academic term. The sample size is 210 students (70 per group). Settings and conduct: The study is conducted at two non-medical faculties of Islamic Azad University, Karaj Branch. Eligible participants are allocated into three groups: intervention one, intervention two, and control. The study is single-blinded and coded so that assessors and analysts are blinded to group allocation. Participants/Inclusion and exclusion criteria: Inclusion criteria: Non-medical students aged 18-25, enrolled at Islamic Azad University, Karaj Branch, written informed consent, access to a smartphone or computer with internet, and the ability to read and write Persian. Exclusion criteria: History of HPV vaccination, known HPV infection or related conditions, participation in HPV education in the last six months, severe psychiatric or neurological disorders, inability to use digital devices. Intervention groups: Intervention Group 1: Digital storytelling education in four sessions over two weeks; each session includes a 5-minute video followed by a 20-minute online Q&A. Intervention Group 2 (Active Control): Traditional education in a single two-hour in-person session, including PowerPoint, images, and a printed pamphlet. Control Group: No training during the study; educational pamphlet provided after study completion. Main outcome variables: Knowledge about human papillomavirus, Attitude towards human papillomavirus, Knowledge about HPV vaccine, Attitude towards HPV vaccine.