Comparing the effectiveness of high-intensity interval training with/without taurine supplementation on the control of oxidative stress indices in female karate athletes

Comparing the effectiveness of high-intensity interval training with/without taurine supplementation on the control of oxidative stress indices in female karate athletes

A single-blind, randomized clinical trial comprising a high-intensity interval training plus taurine supplementation group (n = 10) and a high-intensity interval training plus placebo group (n = 10).

This single-blind clinical trial aims to evaluate female karateka in Kashmar. Twenty participants recruited from local sports clubs will be randomly assigned to two groups of 10: a high-intensity interval training plus taurine group and a HIIT plus placebo group. Participants will be blinded to the capsule contents.

Must have a minimum of two consecutive years of regular karate training experience Must be physically healthy Non-use of any dietary supplements or non-steroidal anti-inflammatory drugs Non-compliance with the prescribed dosage of taurine supplementation or placebo. Non-use of the prescribed dose of taurine supplement or placebo Occurrence of any acute injury or trauma (such as joint or muscle injuries) that prevents the continuation of training

Intervention group (Training with Taurine): The taurine-training group will consume 1 g of taurine (Reyhaneh Pharmaceutical Co., Isfahan, Iran) 45–60 minutes pre-exercise for eight weeks. Concurrently, participants will undergo eight weeks of HIIT (3 sessions/week, 30–35 min/session), starting at 85–90% HRmax and progressing to 90–100% {HRmax} by week eight. The protocol and additional training principle will be conducted in Kashmar, Iran.

Malondialdehyde, Superoxide dismutase enzyme, Total antioxidant capacity

This study will utilize a block randomization method with variable block sizes (e.g., 4 or 6) to allocate participants into two groups: 1) moderate-intensity interval training combined with taurine supplementation, and 2) training combined with placebo. The random sequence will be generated using Random Allocation Software (version 2.0) by an independent researcher not involved in recruitment or intervention, ensuring strict allocation concealment and minimizing selection bias.

The present study is a single-blind clinical trial in which the blinding applies to the taurine and placebo groups. During the first session, participants will read the study protocol. The supplement and placebo are provided in identical capsules distributed among the participants. Only the researcher is aware of the contents of the capsules.